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Research Article | | Peer-Reviewed

Assessment of the Microbial and Physicochemical Quality of Water from Open Wells in the Sagnarigu Municipality in Northern Ghana

Abdallah Yakubu*,  Zangu Daniel Banaamwine,  Banuha Abdulai
Published in Journal of Water Resources and Ocean Science (Volume 15, Issue 2)
Received: 25 February 2026     Accepted: 9 May 2026     Published: 18 May 2026
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Abstract

Background: Many residents in the Sagnarigu Municipality in Northern Ghana rely on open wells for their daily water needs. However, these water sources remain unregulated, unprotected and there is rarely any study that has assessed the water quality and safety of the open wells in the Municipality. The aim of the study was to assess the microbial and physicochemical water quality and safety of open wells in the Sagnarigu Municipality, Northern Ghana by analyzing the microbial and physicochemical water quality parameters. Method: Eight open wells were purposively sampled and analyzed for their microbial (i.e. total coliforms and E. coli) and physicochemical (e.g. pH, turbidity, anions, trace metals, etc.) parameters using standard analytical protocols. The water quality parameters analyzed were compared with Ghana Standard Authority (GSA) and WHO standard measures. Results: The results showed that the microbial quality of the studied open wells were highly compromised as the total coliforms and E. coli were detected. The microbial loads exceeded the WHO and GSA acceptable limit of 0 cfu/100 mL, with values ranging from 5-60 cfu/100 mL for total coliforms and 2.5-80 cfu/100 mL for E. coli. The assessment of the physicochemical parameters revealed that turbidity, TDS, nitrate, nitrite, ammonium and lead exceeded the WHO and GSA standards in some wells, while pH, zinc, mercury, cadmium, arsenic and conductivity were all within the standard measures. Conclusions: The study assessed the microbial and physicochemical quality of water from eight public open wells in the Sagnarigu Municipality, Northern Region, Ghana. The findings showed that none of the studied open wells met the WHO and GSA safety standards. The overall implication of these findings is that the examined open wells present serious public health risks and highlight the urgent need for interventions.

Published in Journal of Water Resources and Ocean Science (Volume 15, Issue 2)
DOI 10.11648/j.wros.20261502.11
Page(s) 29-39
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

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Copyright © The Author(s), 2026. Published by Science Publishing Group
Previous article
Keywords

Microbial Quality, Open Wells, Physicochemical Parameters, Northern Ghana, E. coli, Total Coliforms, Water Quality

1. Introduction
Water is an essential resource for maintaining human life and playing a key role in agriculture, energy, and economic activities. Additionally, it plays a fundamental role in the functioning of a healthy ecosystem
[1]
. Water for domestic use must be clean, safe, affordable, and accessible to every human society to promote healthy living standards and empowerment. Unfortunately, about 2.1 billion people globally still lack access to safe drinking water according to a report by WHO and UNICEF joint monitoring programme for water supply, sanitation and hygiene
[2]
. Ensuring access to clean and safe water requires both global and local efforts. The United Nations recognizes this through its resolution on the human right to safe drinking water and sanitation, which emphasizes that water for drinking and sanitation must be safe, affordable, acceptable, accessible, and available
[3]
. The lack of access to safe drinking water and sanitation services has been linked to various forms of diseases, including diarrhea, dengue, cholera, and soil-transmitted helminth disease
[4]
.
Surface water bodies are natural freshwater sources that supply water for domestic, agricultural and industrial uses
[5]
. Groundwater usually drawn up from boreholes and wells is also an important source of water serving many rural and peri-urban communities in developing countries
[6]
. However, the water quality and safety of these sources are continually being threatened and compromised by population growth, urbanization, industrialization, poor sanitation infrastructure and practices, agricultural activities, climate change, natural and other human activities that lead to contamination and pollution of the water ecosystems
[7, 8]
. Physicochemical contaminants such as trace metals (e.g. Cd, Hg, Pb, etc.) and biological pollutants (e.g. bacteria, protozoa, etc.) can pose significant health risks, including infections, typhoid, gastrointestinal conditions, cancer, kidney disease, liver damage, and neurological disorders. Evaluating heavy metals in drinking water is crucial because it provides insights into the health risk associated with harmful contaminants such as lead, arsenic, mercury, and cadmium
[9-12]
. The degrading water quality and safety is a serious concern and has become a global challenge that requires urgent interventions
[13]
.
Groundwater drawn up from open wells serves as a major source of water for many residents in Ghana and in particular, the Sagnarigu Municipality in the Northern Region. However, these open wells remain largely unregulated and unprotected with significant risk of contamination from surface runoffs, poor sanitary environments and human activities
[14]
. Abdul S. et al. (2025) conducted a case study risk assessment of hand-dug well water in Aflao in Ghana by analyzing heavy metal and microbial contamination. The findings revealed that the heavy metal contents were below detection limits with the microbial loads exceeding the WHO guidelines
[14]
. Iddrisu et al. (2024) in a recent study conducted in the Nanton District, Northern Ghana, reported that approximately 83.3% of 30 groundwater samples analyzed had their physicochemical parameters falling within WHO guidelines but with a widespread microbial contamination in all the samples. Parameters such as iron, manganese, color, turbidity, nitrates, sulfate, and pH were identified as potential influencers of microbial survival in the groundwater
[15]
. In a similar study, Iddrisu et al. (2023) assessed groundwater in Talensi District in the Upper East Region, Ghana. The results of the study revealed that the physicochemical parameters were within WHO guidelines but identified coliform bacteria in several boreholes and wells
[16]
.
There is limited research that has assessed the water quality and safety from open wells in the Sagnarigu Municipality. This gap in knowledge leaves the residents in the Municipality exposed to potential health crises linked to poor water quality and safety. Water quality is a key indicator of its suitability and safety for human use, and its assessment must involve the determination of the microbial and physicochemical parameters that are markers of water contamination and pollution. A systematic assessment of the microbial and physicochemical water quality parameters is therefore essential to safeguard public health safety and guide future water management policies in the Municipality. The main purpose of the study was to assess the quality and safety of water drawn from open wells in the Municipality by analyzing the microbial and physicochemical parameters using WHO and Ghana Standard Authority (DGS 175: 2021) benchmarks. The present study is the first systematic evaluation of the water quality and safety of open wells in the Sagnarigu Municipality and would provide evidence for urgent public health interventions by local authorities and policy decision-makers.
2. Materials and Methods
2.1. Study Area Description
The study was conducted to evaluate the microbial and physicochemical quality of water drawn from open wells located within the Sagnarigu Municipality in the Northern Region of Ghana. The Sagnarigu Municipality is a peri-urban district which lies between latitudes 9°16’ and 9°34’ North and longitudes 0°36’ and 0°57’ West with its Administrative capital being Sagnerigu. It covers a total land area of 200.4 km2 with a population size of 341,711 based on the Ghana Population and Housing census 2021. It shares borders with the Tamale Metropolis to the East and South, Tolon District and Kumbungu District to the West and Savelugu Municipality to the North. It has seen rapid urbanization in recent years. The Municipality comprises a blend of residential communities, informal settlements, farmlands, and semi-rural zones, which makes it a critical area for examining water quality challenges in the Municipality
[17]
. Water sources in the Municipality, particularly open wells, remain vital for household use, especially in areas where piped water infrastructure is inadequate or unreliable. The continued reliance on these wells, many of which are unprotected, raises concerns of potential contamination from nearby pit latrines, surface runoff, animal activity, refuse dumps, and other anthropogenic influences
[18]
.
2.2. Sampling of Open Wells
The sampling of the open wells was guided by factors such as proximity to human and animal activities, sanitation infrastructure, waste disposal practices, population density, and other observed environmental conditions at the sites. Each location was geotagged and documented with field-style environmental observations to complement laboratory findings on microbial and chemical parameters. This study area, with its unique blend of rapid development, traditional practices, and limited access to safe water infrastructure, offers a representative case for understanding the public health implications of groundwater contamination in northern Ghana. A total of eight (8) open wells were purposively sampled and designated as Well A, B, C, D, E, F, G, and H across different communities within the Municipality.
2.3. Water Sample Collection
The water samples were collected in the morning hours (8: 00 – 10: 00 am GMT) as a single event during the onset of the rainy season in April 2025. Eight water samples were collected from the designated wells (Well A to H) based on the standard protocol outlined in the Standard Methods for the Examination of Water and Wastewater by the American Public Health Association. The water samples were collected in clean, sterile, wide-mouth, nonreactive plastic bottles specifically designed for microbiological use. Each container was fitted with a non-leaking cap and a non-toxic liner capable of withstanding repeated sterilization, thereby ensuring that the integrity of the samples was maintained during transport and storage below 10 oC in ice chest. Before water sample collection, dechlorination was performed to neutralize halogens and prevent microbial activity during transit. This ensured that the microbiological composition of the samples remained unaltered before laboratory analysis
[19]
.
2.4. Analysis of Water Quality Parameters
The analyses of the water quality parameters were conducted on each open well water sample within 24 hours after sample collection using calibrated and certified equipment in the Environmental Quality Laboratory of the Council for Scientific and Industrial Research, Water Research Institute, Tamale. Microbiological and physicochemical parameters of the water samples were analyzed to assess the water quality and safety of the open wells in the Sagnarigu Municipality. The analytical results were compared with the standard benchmarks of WHO and Ghana Standard Authority (DGS 175: 2021)
[20, 21]
.
2.5. Analysis of Microbial Quality
Microbial quality is a critical parameter of water potability and safety. It is assessed by analyzing the microbial loads of Escherichia coli and total coliforms as the critical indicators of faecal contamination of water. E. coli and total coliforms represent the density of the bacterial population in drinking water and are the most determined indicators to measure pollution level and water quality
[22]
. The microbial quality of the water samples was assessed within 24 hours of collection by testing E. coli and total coliforms using standard total coliform fermentation technique and membrane filtration technique respectively
[19, 23]
. The resultant colonies per 100 mL of samples were counted using a digital illuminated colony counter to ensure accuracy in the enumeration.
2.6. Analysis of Physicochemical Parameters
The physicochemical parameters of the water samples were analyzed according to the standard methods for the examination of water and wastewater by the American Public Health Association
[19]
. The physicochemical parameters analyzed were pH, turbidity, total dissolved solids (TDS), conductivity, nitrate, nitrite, ammonium, and heavy metals (e.g. Pb, Zn, Cd, etc.).
For the analysis of the physical parameters, specific instruments were employed to ensure accuracy and reliability. The measurement of pH was carried out using the Hanna HI98191 pH/ORP/ISE Meter. Turbidity was determined by the Hach 2100Q Portable Turbidimeter. Total dissolved solids (TDS) were measured using the HM Digital COM100 TDS Meter. Electrical conductivity was analyzed using Eutech CON700 Conductivity Meter.
The chemical analysis of inorganic contaminants in the water samples was conducted using rigorously standardized methods to ensure accuracy and reliability of the results. Nitrate concentrations were assessed through UV-visible spectrophotometry, specifically at 220 nm and 275 nm. This procedure followed the standard methods for the examination of water and wastewater (APHA)
[19]
. The use of dual wavelengths allowed for the correction of potential interferences, thereby improving precision in nitrate quantification. Nitrite determination was carried out using the colorimetric method at a wavelength of 543 nm. This approach relies on the formation of a colored azo dye complex, the intensity of which correlates directly with nitrite concentration, providing a sensitive and specific means of detection. Ammonium levels were quantified using Nessler’s reagent method, in which ammonium ions react with Nessler’s reagent to form a yellow-brown complex. The reagents used were of analytical grade, procured from Sigma Aldrich, ensuring that the sensitivity and accuracy of the method were maintained in line with laboratory best practices.
Heavy metals, including lead (Pb), zinc (Zn), cadmium (Cd), arsenic (As), and mercury (Hg), were analyzed using atomic absorption spectrophotometry (AAS). The measurements were conducted on a PerkinElmer AAnalyst 400 Atomic Absorption Spectrometer (USA). This instrument, equipped with a flame atomization system, allowed for the precise detection of trace metal concentrations. The analysis adhered to APHA Method 3111 B (Flame AAS), which is a globally recognized standard for heavy metal analysis in environmental samples. Before the heavy metal analysis, the samples were filtered with Whatman 0.45 μm paper and reacted with 10% HNO3 to deactivate the activities of microbes and preserve the ions in solution
[24]
.
3. Results and Discussion
The results of microbial and physicochemical parameters of the eight (8) sampled wells in the Sagnarigu Municipality are presented and discussed. The microbial loads of total coliform and E. coli in the sampled wells reveals faecal contamination and highlight the potential health risks associated with the waterborne diseases. The physicochemical parameters are essential for assessing not only the overall safety of drinking water but also its aesthetic quality. The results of the present study is consistent with the findings reported by Iddrisu et al. (2024) and Abdul S. (2025) which both reported widespread microbial contamination with microbial loads exceeding WHO guidelines and physicochemical parameters being within the guidelines
[14, 15]
.
3.1. Microbial Water Quality
Table 1 and Figure 1 display the total coliform and Escherichia coli counts across all eight wells, assessed using APHA Standard Methods 9222A and 9260F, respectively
[25]
. The results of the microbial analysis of the water samples revealed widespread contamination across all the sampled wells. The microbial loads far exceeded the WHO and GS acceptable limit of less than 1 cfu/100 mL for both E. coli and total coliforms, with values ranging from 2-60 cfu/100 mL for total coliforms and 5-80 cfu/100 mL for E. coli. These figures are alarmingly high, considering the maximum allowable limits set by both WHO and GSA. This clearly indicates severe faecal contamination, likely due to poor sanitation infrastructure, proximity of the wells to pit latrines, or surface water runoff during rains and highlights a significant public health concern. Among the wells, Wells F and D presented the highest risk, recording the most alarming microbial loads. Such elevated levels strongly suggest the influence of nearby contamination sources, including latrines, surface runoff, or animal waste. In contrast, Wells G and H had relatively lower microbial loads compared to the others. However, their values still exceeded the permissible limits and remained microbiologically unsafe with potential health risks. With these high levels of microbial loads of total coliform and E. coli, the residents in the Municipality are exposed to potential health crises and are likely to be infected with waterborne diseases such as typhoid, cholera, diarrheal diseases and among others
[26]
.
Figure 1. Microbial load of total coliform and E. coli in the water samples from Wells A-H compared with WHO and GSA standards.
Table 1. Microbial Quality of Water Samples from Wells A–H compared with WHO and GSA standards.

Sampled Wells

E. coli (cfu/100 mL)

Total Coliform (cfu /100 mL)

GSA (DGS 175: 2021) cfu/100 mL

WHO Guideline cfu/100 mL

Well A

20

54

<1

<1

Well B

5

34

<1

<1

Well C

12

18

<1

<1

Well D

48

71

<1

<1

Well E

28

36

<1

<1

Well F

60

80

<1

<1

Well G

2

5

<1

<1

Well H

9

10

<1

<1
3.2. Physicochemical Water Quality
The physicochemical quality parameters analyzed for the water samples were compared with the WHO and GS standard benchmarks and the results are presented in Table 2 with the summary descriptive statistics presented in Table 3. The analysis of the physicochemical quality of the sampled wells revealed several notable patterns.
3.3. pH of Water Samples
pH is an essential parameter in water quality assessment. pH of water determines the solubility of chemical constituents such as nutrients and heavy metals and biological availability
[26]
. As seen in Table 2 and Figure 2A, the pH values of the Wells ranged between 6.60–7.30 and were all found to be within the WHO and GS acceptable range of 6.5–8.5, indicating neutral water quality. While pH is not a direct toxicological parameter, it is an operational lever in that water with less than pH 6.5 is more corrosive, which can enhance the leaching of toxic metals such as lead, cadmium or copper from plumbings and mineral rocks into the water. Water with pH higher than 8.0 can drive scaling and affect disinfectant performance
[27]
.
3.4. Conductivity Measurement
The conductivity value is an index that represents the concentration of soluble salts that affect the taste of drinking water source
[23]
. From Table 2 and Figure 2B, Well F had the highest electrical conductivity and Well D recorded the least conductivity compared to the other wells. However, the electrical conductivity levels recorded were below the 1,500 μS/cm limit outlined by the WHO and GSA guidelines, thus showing that the electrolytes in the water samples were relatively low
[24]
. The conductivity of water has no direct health risk, but high conductivity indicates the addition of some pollutants to it
[27]
.
3.5. Turbidity Measurement
Turbidity is a critical parameter as it can interfere with disinfection processes and provide a medium for microbial growth
[28]
. From Table 2 and Figure 2C, the turbidity levels in Wells A, B, C, D, and F exceeded the WHO and GSA guideline values of 5 NTU with Well G barely exceeding the limit
[21, 29]
. Wells E and H were within the acceptable measure of water quality. The exceedances observed across multiple wells suggest widespread contamination, possibly due to poor well protection, proximity to latrines, surface runoff intrusion, agricultural runoff, water drawing containers, or unlined well walls that allow surface water intrusion. Turbidity not only reduces aesthetic acceptability making water appear cloudy or unclean but also indicates that the microbial risk is likely underestimated, since particulate matter can harbor bacteria and viruses
[29]
.
Elevated turbidity indicates the presence of suspended particles such as clay, silt, organic matter, and microorganisms that impair water clarity. From a public health standpoint, this is of great concern because high turbidity can shield pathogenic organisms like E. coli and other enteric pathogens from disinfection, thereby increasing the risk of waterborne disease transmission
[30]
. Given that clear water is critical for both acceptability and safety, the observed turbidity levels highlight the urgent need for improved construction, regular cleaning, and community-level interventions such as fencing of wells, proper drainage, and covering of openings. In addition, treatment methods like sedimentation, filtration, or boiling may be required at the household level to mitigate health risks.
3.6. Total Dissolved Solids in Water Samples
TDS is a measure of the total dissolved solids in water that comprises inorganic salts, principally calcium, magnesium, potassium, sodium, bicarbonates, chlorides and sulfates, and small amounts of organic matter that are dissolved in water. TDS in drinking-water originates from natural sources, sewage, urban runoff, and industrial wastewater. Concentrations of TDS in water vary considerably in different geological regions owing to differences in the solubility of minerals
[29]
. As can be referenced from Table 2 and Figure 2D, the levels of TDS in Wells B, D, E, F, and H) were above the GSA limit of 500 mg/L. The TDS in Wells C and G were within the GSA limit with Well A barely exceeding the limit. While this is of concern from a national standards perspective, it is important to note that all the recorded TDS values were still below the WHO threshold of 1000 mg/L and may not pose an immediate health risk. This indicates that although the water may not fully comply with local standards, it remains within the broader international guideline and may not pose an immediate health risk. The exceedance of DGS 175: 2021 suggests that local geological formations or anthropogenic activities (e.g., agricultural runoff, waste leaching) are contributing to mineral loading in Wells B, D, E, F, and H. The fact that all wells remain within WHO limits suggests that the water may still be consumable but requires monitoring and possible treatment (e.g., reverse osmosis or activated carbon filtration) to improve acceptability and compliance with national standards
[31]
. From a public health perspective, no health-based guideline values have been proposed for TDS by the WHO. Water with a TDS content of less than about 600.00 mg/L is generally considered as good. However, high TDS content can significantly cause undesirable taste, hardness, and scaling properties to water, making it less acceptable for domestic use
[22, 24]
.
3.7. Nitrate and Nitrite Content in Water Samples
Nitrate and nitrite concentrations were another area of concern, Nitrate and nitrite contaminations are often associated with agricultural runoff, particularly from fertilizer use or leachate from poorly managed sanitation systems. The level of nitrate and nitrite in drinking water causes diseases such as blue baby syndrome, cancer and bleeding of spleen
[5, 23]
.
From Table 2 and Figure 2E, the results show that nitrate concentrations in the wells ranged from 2.35 mg/L to 11.25 mg/L. According to both the WHO and GSA, the permissible limit for nitrate in drinking water is 50 mg/L. All the wells had nitrate content below the permissible limits. Elevated nitrate levels in groundwater are often associated with agricultural runoff, improper waste disposal, and leaching from latrines or septic systems. Chronic exposure to high nitrate concentrations is linked to methemoglobinemia or “blue baby syndrome” in infants, as well as possible long-term risks of certain cancers due to endogenous formation of N-nitroso compounds. The detection of elevated levels in some wells highlights the vulnerability of shallow groundwater sources in peri-urban and rural areas where agriculture and poor sanitation are common
[32, 33]
.
In Table 2 and Figure 2F, the observed nitrite concentrations ranged from 0.0065 mg/L to 1.55 mg/L. WHO and GSA both set a guideline limit of 3.0 mg/L for nitrite in drinking water. The nitrite contents were within the acceptable standards for the Wells except for Well B which had the highest of 1.55 mg/L. Nitrite is more toxic than nitrate and poses immediate health risks because it directly oxidizes hemoglobin to methemoglobin, impairing oxygen transport in the blood
[34]
. The exceedances observed in Wells B suggest possible microbial contamination, as nitrite is typically an intermediate product of nitrification and denitrification processes in groundwater
[35]
. Its presence above the permissible limit is particularly concerning, since nitrite is unstable and usually indicates active pollution, often from faecal matter or organic waste.
Overall, the results highlight the importance of continuously monitoring both nitrate and nitrite levels in drinking water sources. While most wells are within acceptable standards, the exceedances in certain wells reflect localized contamination risks. These findings also highlight the need for enhanced groundwater protection strategies, including the regulation of agricultural inputs, improvements in sanitation, and community water safety planning in the Sagnarigu Municipality.
3.8. Ammonium Content in Water Samples
From Table 2 and Figure 2G, the ammonium concentrations ranged from 0.001 to 1.6 mg/L, with Wells C, D, E, and G exceeding both WHO and GSA standards of 0.2 mg/L and 0.5 mg/L respectively. The ammonium content in Wells A and B were below both the WHO and GSA standards. Well F had its ammonium content exceeding the WHO limit but equaled the GSA limit. In Well H, the ammonium content exceeded the WHO limit but was below the GSA limit. Ammonium can occur naturally in water supplies. Sewage contains large amount of ammonia formed by bacterial decay of nitrogenous organic wastes. It is an indicator of possible bacterial, sewage and animal waste pollution
[27]
. Ammonium itself is not generally a direct toxicological concern at the concentrations encountered in drinking water; instead, it is valued as a diagnostic indicator of recent pollution (e.g., sewage intrusion, manure, or fertilizer inputs) and of potential chloramine formation issues during disinfection
[20, 29]
.
3.9. Heavy Metal Contents in Water Samples
Heavy metals are metallic elements with a specific weight greater than 5 g/cm3 and are toxic at lower concentrations. Heavy metals comprised of the essential metals (Cu, Zn, Co, Cr, Mn, and Fe), non-essential metals (Ba, Al, Li, and Zr), less toxic metals (Sn and As), and highly toxic metals (Hg, Cd, and Pb). The presence of these toxic metals suggests either geogenic leaching from underlying rock formations or anthropogenic contamination, potentially from industrial effluents, improper waste disposal, or corroded pipelines
[36]
. Assessment of the adequacy of the heavy metals content in drinking-water relies on comparison of the results of water quality analysis with guideline values
[20]
.
3.10. Lead Content in Water Samples
As can be seen in Table 2 and Figure 2H, lead (Pb) was detected in all the wells with its concentration within the 0.01 mg/L permissible limits for drinking water quality except Well F whose Pb concentration of 0.012 mg/L slightly exceeded both WHO and GSA standards. WHO emphasizes there is no known safe threshold of Pb for key neurodevelopmental effects, particularly in infants and young children, and retains 0.01 mg/L as a provisional target because achieving lower levels can be challenging with centralized conditioning alone. The exceedance observed in Well F is minor, but it still warrants attention, with priority given to source tracing and exposure control. Prolonged exposure to lead through drinking water is associated with cognitive impairment in children and kidney dysfunction in adults. Corrosion control (managing pH/alkalinity), replacing lead-bearing components and, as an interim measure, certified point of use filters for lead can reduce exposure
[20]
.
3.11. Zinc Content in Water Samples
Zinc concentrations across the wells ranged between 0.005–0.025 mg/L and were all orders of magnitude below the WHO and GSA limit of 3.0 mg/L where aesthetic concerns typically arise. Toxicologically, WHO does not set a health-based guideline for zinc in drinking water quality because zinc intake primarily comes from the diet, and its concentrations in water that could cause health effects are much higher than those found in well-managed supplies
[20]
. The results as presented in Table 2 and Figure 2I, therefore, suggest that Zn presents no health risk or palatability issue.
3.12. Mercury Content in Water Samples
All the wells recorded mercury levels of <0.001 mg/L, which fall below both the stringent WHO guideline but within the GSA threshold as presented in Table 2 and Figure 2J. From a health perspective, mercury in drinking water is mainly a concern in areas affected by mining activities or industrial discharges, since WHO technical documents emphasize its neurotoxicity and link it to global public health priorities such as those outlined in the Minamata Convention. In this case, the non-detections indicate that mercury does not pose an immediate public health concern in the study area. However, it would still be prudent to maintain periodic monitoring, especially if there are artisanal mining activities nearby or if the hydrogeology suggests the potential for long-range transport from upstream sources
[20]
.
3.13. Cadmium Content in Water Samples
As presented in Table 2 and Figure 2K, all the measured Cd values were ≤ 0.002 mg/L and fell below the 0.003 mg/L permissible limit. WHO guidance retains 0.003 mg/L based on kidney toxicity, with β2-microglobulin identified as the critical effect biomarker, and the long biological half-life emphasizes the importance of cumulative exposure control. The pattern of low but detectable results in several wells does not indicate current health concern but does warrant routine monitoring, as cadmium inputs may originate from phosphate fertilizers, waste disposal, or specific industrial sources
[20, 37]
.
3.14. Arsenic Content in Water Samples
As can be seen in Table 2 and Figure 2L, all the wells had arsenic content below 0.01 mg/L, the widely used risk-based goal that WHO designates as provisional given treatment and quantification constraints in many small systems. Arsenic is a potent chronic carcinogen with skin, vascular, and internal cancer endpoints; hence, even low but near threshold concentrations are taken seriously worldwide. The uniformly low values are reassuring and, in a Ghanaian context, suggest either favorable local geology or effective source protection because certain gold bearing formations can yield elevated geogenic arsenic. Continued periodic monitoring remains important due to spatial heterogeneity and seasonal shifts
[20, 37]
.
Table 2. Physicochemical water quality parameters of open wells.

Parameter

Unit

WHO Limit

GSA Limit

Well A

Well B

Well C

Well D

Well E

Well F

Well G

Well H

Conductivity

µS/cm

1500

1500

985

885

762.5

622.5

740

1020

695

835

Turbidity

NTU

5.0

5.0

5.25

5.20

5.45

5.65

4.8

6.5

5.1

5.0

pH

units

6.5–8.5

6.5–8.5

7.15

7.145

7.06

6.95

7.30

6.60

6.75

7.25

TDS

mg/L

1000

500

507.5

607.5

499.5

521

612

700

485

530

Nitrate

mg/L

50.0

50.0

2.35

3.55

11.25

2.85

6.75

9.25

10.8

3.3

Nitrite

mg/L

3.0

3.0

0.0065

1.55

0.0095

1.25

0.8

0.5

1.1

0.3

Ammonium

mg/L

0.2

0.5

<0.001

<0.089

1.255

0.955

0.75

0.5

1.6

0.45

Lead

mg/L

0.01

0.01

<0.005

<0.005

0.0085

<0.001

0.007

0.012

0.003

0.006

Zinc

mg/L

3.00

3.00

0.006

0.0085

0.015

0.007

0.010

0.025

0.018

0.005

Mercury

mg/L

0.006

0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

Cadmium

mg/L

0.003

0.003

0.0015

0.001

<0.002

<0.002

0.002

0.0015

<0.002

0.001

Arsenic

mg/L

0.01

0.01

0.001

0.001

<0.001

<0.001

0.002

0.0015

<0.001

0.001
Figure 2. Physicochemical water quality parameters of open wells.
Table 3. Summary of Descriptive Statistics of Physicochemical Parameters in Wells A–H.

Parameter

Min

Max

Mean

SD

WHO/GSA Limit

Conductivity (µS/cm)

622.5

1020

818.75

±121.2

1500

Turbidity (NTU)

4.8

6.5

5.37

±0.49

5.0

pH

6.60

7.30

7.04

±0.23

6.5–8.5

TDS (mg/L)

485

700

570.56

±73.7

1000/500

Nitrate (mg/L)

2.35

11.25

6.65

±3.10

50.0

Nitrite (mg/L)

0.0065

1.55

0.777

±0.61

3.0

Ammonium (mg/L)

0.001

1.6

0.626

±0.52

0.2/0.5

Lead (mg/L)

0.001

0.012

0.0059

±0.0043

0.01

Zinc (mg/L)

0.005

0.025

0.011

±0.0067

3.00

Mercury (mg/L)

0.001

0.001

0.001

0

0.006/0.001

Cadmium (mg/L)

0.002

0.002

0.0012

±0.00058

0.003

Arsenic (mg/L)

0.001

0.002

0.0010

±0.00063

0.01
4. Implications for Policy and Practice
The overall implication of these findings is that none of the wells examined is suitable for direct consumption without prior treatment, particularly disinfection. The dual burden of microbial and chemical pollution presents a serious public health risk and highlights the urgent need for intervention. From a policy perspective, the situation calls for the enforcement of well construction regulations, including proper lining and covering, as well as the institution of regular water quality monitoring, especially in peri-urban communities. In addition, there is a pressing need for public awareness campaigns focused on environmental sanitation and the adoption of household-level water treatment technologies such as chlorination and filtration. Collectively, these findings should guide the development of regional water safety plans and provide a foundation for future research aimed at strengthening community water system improvements
[38]
.
5. Conclusion
This study provides the first assessment of the microbial and physicochemical quality and safety of water from eight public open wells in the Sagnarigu Municipality, Northern Region of Ghana. The findings showed a widespread microbial contamination, with E. coli and total coliform counts far exceeding WHO and Ghana Standards Authority (DGS 175: 2021) limits, indicating severe faecal and organic matter pollution. Several physicochemical parameters, including turbidity, total dissolved solids, ammonium, and lead exceeded WHO and GSA benchmarks, further compromising the water safety. Overall, it is evident none of the open wells have met the complete safety standards for direct domestic use and therefore presents a serious public health risk. This highlights the urgent need for well protection, household-level treatment, regular water quality monitoring, public awareness education on water safety measures and environmental sanitation, and policy interventions. This will safeguard public health and ensure that residents in the Sagnarigu Municipality have access to safe drinking water. Future research should extend the study to cover seasonal variations, a wider geographic catchment area, intervention studies, parasitological analysis, analysis of emerging contaminants such as microplastics and per- and polyfluoroalkyl substances (PFAS) in groundwater and assessment of health outcomes associated with water quality.
6. Limitations of the Study
The findings are limited and indicative only to the eight (8) open wells purposively sampled and cannot be generalized to the water quality and safety of open wells in the Sagnarigu Municipality given the potential biases associated with purposive sampling. Also, seasonal variations (rainy and dry) and replicate measurements were not considered in the study design. Insufficient time and funding problems also impacted on the study scope and outcomes.
Abbreviations

APHA

American Public Health Association

DGS

Draft Ghana Standards

GSA

Ghana Standard Authority

NTU

Nephelometric Turbidity Unit

TDS

Total Dissolved Solids

WHO

World Health Organization
Acknowledgments
We acknowledge the support of the staff at the Council for Scientific and Industrial Research, Water Research Institute, Tamale for availing their laboratory for the analysis.
Author Contributions
Abdallah Yakubu: Conceptualization, Project administration, Supervision, Resources, Data curation, Validation, Writing – original draf, Writing – review & editing
Zangu Daniel Banaamwine: Investigation, Methodology, Data curation, Visualization, Formal Analysis, Writing – review & editing
Banuha Abdulai: Investigation, Methodology, Data curation, Visualization, Formal Analysis, Writing – review & editing
Funding
This study was not funded by any governmental or non-profit organization.
Data Availability Statement
All relevant data have been presented in this manuscript or it’s Supplementary Information. Any additional data or information needed regarding this research can be sourced from the corresponding author.
Conflicts of Interest
The authors declare no conflicts of interest in relation to this work.
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    Yakubu, A., Banaamwine, Z. D., Abdulai, B. (2026). Assessment of the Microbial and Physicochemical Quality of Water from Open Wells in the Sagnarigu Municipality in Northern Ghana. Journal of Water Resources and Ocean Science, 15(2), 29-39. https://doi.org/10.11648/j.wros.20261502.11

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    Yakubu, A.; Banaamwine, Z. D.; Abdulai, B. Assessment of the Microbial and Physicochemical Quality of Water from Open Wells in the Sagnarigu Municipality in Northern Ghana. J. Water Resour. Ocean Sci. 2026, 15(2), 29-39. doi: 10.11648/j.wros.20261502.11

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    AMA Style

    Yakubu A, Banaamwine ZD, Abdulai B. Assessment of the Microbial and Physicochemical Quality of Water from Open Wells in the Sagnarigu Municipality in Northern Ghana. J Water Resour Ocean Sci. 2026;15(2):29-39. doi: 10.11648/j.wros.20261502.11

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  • @article{10.11648/j.wros.20261502.11,
  author = {Abdallah Yakubu and Zangu Daniel Banaamwine and Banuha Abdulai},
  title = {Assessment of the Microbial and Physicochemical Quality of Water from Open Wells in the Sagnarigu Municipality in Northern Ghana},
  journal = {Journal of Water Resources and Ocean Science},
  volume = {15},
  number = {2},
  pages = {29-39},
  doi = {10.11648/j.wros.20261502.11},
  url = {https://doi.org/10.11648/j.wros.20261502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wros.20261502.11},
  abstract = {Background: Many residents in the Sagnarigu Municipality in Northern Ghana rely on open wells for their daily water needs. However, these water sources remain unregulated, unprotected and there is rarely any study that has assessed the water quality and safety of the open wells in the Municipality. The aim of the study was to assess the microbial and physicochemical water quality and safety of open wells in the Sagnarigu Municipality, Northern Ghana by analyzing the microbial and physicochemical water quality parameters. Method: Eight open wells were purposively sampled and analyzed for their microbial (i.e. total coliforms and E. coli) and physicochemical (e.g. pH, turbidity, anions, trace metals, etc.) parameters using standard analytical protocols. The water quality parameters analyzed were compared with Ghana Standard Authority (GSA) and WHO standard measures. Results: The results showed that the microbial quality of the studied open wells were highly compromised as the total coliforms and E. coli were detected. The microbial loads exceeded the WHO and GSA acceptable limit of 0 cfu/100 mL, with values ranging from 5-60 cfu/100 mL for total coliforms and 2.5-80 cfu/100 mL for E. coli. The assessment of the physicochemical parameters revealed that turbidity, TDS, nitrate, nitrite, ammonium and lead exceeded the WHO and GSA standards in some wells, while pH, zinc, mercury, cadmium, arsenic and conductivity were all within the standard measures. Conclusions: The study assessed the microbial and physicochemical quality of water from eight public open wells in the Sagnarigu Municipality, Northern Region, Ghana. The findings showed that none of the studied open wells met the WHO and GSA safety standards. The overall implication of these findings is that the examined open wells present serious public health risks and highlight the urgent need for interventions.},
 year = {2026}
}

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  • TY  - JOUR
T1  - Assessment of the Microbial and Physicochemical Quality of Water from Open Wells in the Sagnarigu Municipality in Northern Ghana
AU  - Abdallah Yakubu
AU  - Zangu Daniel Banaamwine
AU  - Banuha Abdulai
Y1  - 2026/05/18
PY  - 2026
N1  - https://doi.org/10.11648/j.wros.20261502.11
DO  - 10.11648/j.wros.20261502.11
T2  - Journal of Water Resources and Ocean Science
JF  - Journal of Water Resources and Ocean Science
JO  - Journal of Water Resources and Ocean Science
SP  - 29
EP  - 39
PB  - Science Publishing Group
SN  - 2328-7993
UR  - https://doi.org/10.11648/j.wros.20261502.11
AB  - Background: Many residents in the Sagnarigu Municipality in Northern Ghana rely on open wells for their daily water needs. However, these water sources remain unregulated, unprotected and there is rarely any study that has assessed the water quality and safety of the open wells in the Municipality. The aim of the study was to assess the microbial and physicochemical water quality and safety of open wells in the Sagnarigu Municipality, Northern Ghana by analyzing the microbial and physicochemical water quality parameters. Method: Eight open wells were purposively sampled and analyzed for their microbial (i.e. total coliforms and E. coli) and physicochemical (e.g. pH, turbidity, anions, trace metals, etc.) parameters using standard analytical protocols. The water quality parameters analyzed were compared with Ghana Standard Authority (GSA) and WHO standard measures. Results: The results showed that the microbial quality of the studied open wells were highly compromised as the total coliforms and E. coli were detected. The microbial loads exceeded the WHO and GSA acceptable limit of 0 cfu/100 mL, with values ranging from 5-60 cfu/100 mL for total coliforms and 2.5-80 cfu/100 mL for E. coli. The assessment of the physicochemical parameters revealed that turbidity, TDS, nitrate, nitrite, ammonium and lead exceeded the WHO and GSA standards in some wells, while pH, zinc, mercury, cadmium, arsenic and conductivity were all within the standard measures. Conclusions: The study assessed the microbial and physicochemical quality of water from eight public open wells in the Sagnarigu Municipality, Northern Region, Ghana. The findings showed that none of the studied open wells met the WHO and GSA safety standards. The overall implication of these findings is that the examined open wells present serious public health risks and highlight the urgent need for interventions.
VL  - 15
IS  - 2
ER  -

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    1. 1. Introduction
    2. 2. Materials and Methods
    3. 3. Results and Discussion
    4. 4. Implications for Policy and Practice
    5. 5. Conclusion
    6. 6. Limitations of the Study
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